Figure 2. PKC activation by Cr(VI) was independent of Src/Ras signaling. (A) BEAS-2B cells and keratinocytes were treated with Cr(VI) for 2 h or 1 month in the presence or absence of GO6976 (1.0 μM), FTI (0.25 μM) or PP1 (0.15 μM). Afterwards, lysates were prepared for PKC activity assay. The error bars were SD (n = 5, p < 0.05). (B) BEAS-2B cells were exposed to Cr(VI) for 2 h or 24 h in the presence or absence of GO6976 and then assayed for the expressions of the phosphorylated Src by immunoblotting and active Ras using active Ras pull-down kit. Src and actin were the loading controls. (C) Cells were treated as described above and levels of ROS were then analyzed. The error bars were SD (n = 5, p < 0.01).