Figure 5. Role of PKC or Src/Ras signaling in Cr(VI)-mediated transformation. (A) Cells were cultured in soft agar containing Cr(VI) or Cr(VI) plus PP1, FTI or GO6976, respectively for another 4.5 months. Untreated cells grown in soft agar medium served as the control. The numbers of the colonies were counted (left panel) and examples of colonies from the treatments were imaged (right panels). The error bars were SD (n = 5, p < 0.05). (B) Expression of active Ras, phosphorylated c-Jun and Bcl-2 in two Cr(VI) transformed colonies were examined by immunoblotting (left panels). The folds of the proteins induced were measured and then plotted (right panels). Actin was the loading control.