Research Paper Volume 14, Issue 5 pp 2081—2100

Modulation of RNA splicing associated with Wnt signaling pathway using FD-895 and pladienolide B


Figure 3. Intron retention of DNAJB1 gene in different cancer cell lines. Tumor cell lines were treated with 100 nM of FD-895, 100 nM pladienolide B, 30 μM cisplatin or 30 μM etoposide for 4 h. Analysis of IR of DNAJB1 mRNAs was evaluated by RT-PCR in (AC) Mantle cell lymphoma cells, (Jeko-1, Mino and JMV-2), (D) HeLa, (E) MDA-MB-468 (F) HCT116 and (G) MCF-7 cells. RNU6A, an intronless gene was used as RNA quality and loading control.