Priority Research Paper Volume 14, Issue 5 pp 2025—2046

Depletion of transmembrane mucin 4 (Muc4) alters intestinal homeostasis in a genetically engineered mouse model of colorectal cancer


Figure 4. Muc4 deletion results in defective mucus barrier function and reduced intestinal homeostasis molecules. (A) TCGA-COAD dataset showed down-regulation of FAM3D in CRC patients (N = 286) compared with healthy controls (N = 41). (B) Immunohistochemistry analysis of Fam3d expression in the colon of normal, AC, and AMC mice. n = 6 (AC and AMC) and n=3 for normal group. (C) Representative images of immunofluorescent staining of bacteria (red color) done by fluorescence in-situ hybridization using EUB338-Cy3 probe. n = 3 per group. (D) mRNA expression levels of antimicrobial peptides (Reg3b, Reg3γ, and Saa3) in the colons of AC and AMC mice were measured by real-time PCR. n = 3-4 per group.