Priority Research Paper Volume 14, Issue 5 pp 2025—2046
Depletion of transmembrane mucin 4 (Muc4) alters intestinal homeostasis in a genetically engineered mouse model of colorectal cancer
Figure 5. Muc4 deletion results in up-regulation of β-catenin signaling. (A) Immunohistochemical analysis of β-catenin in normal, AC and AMC animals. n = 6 (AC and AMC) and n=3 for normal group. (B) IHC composite score of nuclear β-catenin staining in AC and AMIC mice (C) MUC4 and CTNNB1 (β-catenin) correlated negatively in the TCGA-COAD dataset. (D) Immunoblot of active β-catenin and its target genes: cyclin-D1 and c-Myc expression in AC and AMC animals. n = 3 per group.