Figure 6. Knockdown (KD) of MUC4 in CRC cell lines mediates β-catenin signaling. (A) A panel of CRC cell lines was used and screened for MUC4 expression (Colo357 pancreatic cancer cell line used as a positive control). (B) Stable KD of MUC4 in both LS-180 and HCT-8 CRC cell lines. (C) Expression of nuclear β-catenin levels in a sub-cellular fraction of CRC cell line done by western blot.