Research Paper Volume 14, Issue 5 pp 2287—2303

MafK accelerates Salmonella mucosal infection through caspase-3 activation


Figure 2. MafK leads to abnormal inflammatory responses. Streptomycin-pretreated WT and MafK mice were orally infected with Salmonella (n = 9 each group) for 48 h. The homogenate supernatant of cecum was detected for concentrations of indicated cytokines by ELISA. (A) TNF-α, (B) IL-6, (C) IL-1 β, (D) IFN-γ, (E) IL-10. (F) The homogenate supernatant of the cecum was also used to determine the activity of MPO. (G) Representative cecum tissue lysates were analyzed for iNOS and Cox-2 by western blotting. GAPDH was used as a loading control. (H) Representative immunohistochemical staining of Gr-1 (a neutrophil marker) and F4/80 (a macrophagocyte marker) were performed in the cecal sections. All data are shown as the mean ± SEM. Student’s t test was performed. Statistical significance is indicated by *p < 0.05, **p < 0.01.