Research Paper Volume 14, Issue 5 pp 2287—2303

MafK accelerates Salmonella mucosal infection through caspase-3 activation


Figure 4. MafK increases intestinal barrier damage during Salmonella infection. Streptomycin-pretreated WT and MafK Tg mice were orally infected with Salmonella (n = 10 each group) for 48 h. Claudin3 expression was examined in cecum sections by (A) immunofluorescence and (B) western blotting. The mRNA levels of (C) claudin-3, (D) occludin and (E) ZO-1 in the cecum were examined by real-time PCR. The data were normalized to GAPDH and are shown as the fold increase in mRNA. (F) Immunohistochemistry staining for mucin 2. (G) Intestinal permeability was measured using FITC-dextran. *p < 0.05, **p < 0.01.