Figure 3. The blockade of the cell cycle causes a prelamin A accumulation in immortalized MEF Zmpste24 KO cells. (A) Immunoblot analysis of Lamin A (in MEF Zmpste24 WT cells), prelamin A (in MEF Zmpste24 KO cells), p27 and LC3B II-I ratio using β actin as loading control, in the cell extracts with FBS reduction (n = 3). (B) The plot indicates the quantification data of Lamin A or prelamin A/β-actin ratio, p27/β-actin ratio and LC3B II/LC3B I ratio of MEF Zmpste24 WT and KO in both in growth state and in nutrient deprivation. Data represent the mean ± standard error of the mean (SEM). Differences were determined by Dunnett's multiple comparisons test. *p < 0,05; **p < 0,01 (n = 3).