Figure 4. Verification of CEP290 expression levels in liver cancer cells. (A, B) CEP290 levels in HCC cells were measured by qRT–PCR and WB. (C) The quantification of protein expression of CEP290 in diverse cell lines. (D, E) CEP290 interference reduced mRNA and protein expression in Huh7 cells. (F) Cell proliferation measured by CCK-8 assay after CEP290 interference. (G, H) Representative images (left) and quantification (right) of colony formation capacity. (*p < 0.05; **p < 0.01; ***p < 0.001; ##p < 0.01, ###p < 0.001).