Research Paper Volume 14, Issue 5 pp 2367—2382

Centrosomal protein 290 is a novel prognostic indicator that modulates liver cancer cell ferroptosis via the Nrf2 pathway


Figure 5. Effect of CEP290 interference on liver cancer cell ferroptosis and malignant phenotypes. (A) Scratch assay. Scratches were made in a confluent culture, and cell migration into the scratch wounds was documented. (B, C) Transwell assays. After treatment, the migration and invasion of Hep3B cells were analyzed. (D) Hep3B cell viability was evaluated after si-CEP290, incorporate with treatment of Z-DEVD-FMK (100 μM), 3-MA (10 mM), and ferrostain-1 (100 μM). (EG) Cell viability, ferrous iron and MDA levels in Hep3B cells treated with si-CEP290 and 75 μM oltipraz (* si-CEP290 vs. si-CEP290+oltipraz, **p < 0.01, *p < 0.05; # si-NC vs. si-CEP290, ##p < 0.01, #p < 0.05).