Research Paper Volume 14, Issue 5 pp 2400—2417

Figure 5. MT exerted its effects through regulating the SIRT1/FOXO1 pathway. (A) Immunofluorescence staining showed the expressions of SIRT1 (red fluorescent) and ac-FOXO1 (green fluorescent) (bar = 50 μm). The white arrow indicates that the two proteins were co-expressed in the NPCs. (B, C) The fluorescence intensity of SIRT1 and ac-FOXO1 was analyzed. (D–G) A Western blot analysis showed the relative expressions of SIRT1, FOXO1 and ac-FOXO1 proteins in NPCs. (H, I) PCR results showed the relative mRNA expressions of SIRT1 and ac-FOXO1. Values are expressed as the mean ± SD (N = 8). Compared with the YCG, ^##P < 0.01; compared with the AG, ^**P < 0.01. SIRT1: silent mating type information regulation 2 homolog-1, FOXO1: forkhead box O1, YCG: young control group, AG: aging group, MTG: manual therapy group.