Research Paper Volume 14, Issue 6 pp 2590—2606

The TLR4/NF-κB/MAGI-2 signaling pathway mediates postoperative delirium


Figure 6. Proteomics-based validation of human peripheral blood. Based on the results of DIA proteomics and the hypothesis of this study, peripheral blood on the third postoperative day (T2) from eight POD patients and eight non-POD patients with DIA proteomics was validated by western blot analysis for selected differential proteins and the TLR4/NF-κB signaling pathway (A). The expression of MAGI-2 in the peripheral blood of POD patients was significantly higher than that of non-POD patients (B). TLR4/NF-κB pathway-related protein expression is shown in Figure 6B. Compared with non-POD patients, p-NF-κB-p65 and TLR4 were highly expressed in POD patients (B). POD: patients with postoperative delirium; non-POD: patients without postoperative delirium. Compared with non-POD group, *p<0.05.