Figure 5. Administration of THSG decreased TBI-induced neural apoptosis in the brain cortex post TBI. (A) Schematic illustration of regions of interest (ROIs) in cerebral cortex with sham (left) or TBI (right). The sampling areas are shown in red squares. (B) Representative double staining immunofluorescence with TUNEL assay (green) and NeuN (a maker for neurons, red) and DAPI (blue) counterstain in brain cortex from the Sham + vehicle, Sham + THSG, TBI + vehicle, and TBI + THSG group. Labeling co-localization is shown as yellow in the merged images. Scale bar = 50 μm. (C) Quantification of the co-localization of the TUNEL positive and NeuN, as well as the counterstaining with DAPI in cortical brain tissues from the Sham + vehicle, TBI + vehicle, and TBI + THSG groups. Data are expressed as the mean ± SEM (n = 6 per group). ***, P < 0.001 between groups.