Research Paper Volume 14, Issue 6 pp 2695—2719

Effects of caloric overload before caloric restriction in the murine heart


Figure 7. Expression of Adiponectin and Adiponectin receptors. mRNA levels of Adiponectin in WHT (A). Immunoblot results (B) and protein expression of Adiponectin (C). mRNA expression of the Adiponectin receptors AdipoR1 (D), AdipoR2 (E) and T-cadherin (F). The transcripts were analyzed by qPCR. Significant differences between groups are expressed as % values. For each gene expressed as %, the 3.1CG value was set to 0, and the values of the compared samples were normalized to this level. Positive values represent upregulation. Negative values represent downregulation. Each marker was analyzed with SYBR Green fluorescence detection, and the transcript levels of the target genes were normalized to those of the endogenous control 18S rRNA. Adiponectin protein expression was obtained by Western blot analysis and quantified with Image Lab 6.1 software. The values were normalized to GAPDH expression; the 3.1CG expression was set to 1.0. The data are the mean ± SD. *P < 0.05; **P < 0.01 vs. the 3.1CG group, unless otherwise specified.