Research Paper Volume 14, Issue 7 pp 3070—3083
Hypoxic pretreatment of adipose-derived stem cell exosomes improved cognition by delivery of circ-Epc1 and shifting microglial M1/M2 polarization in an Alzheimer’s disease mice model
Figure 5. Overexpressing miR-770-3p or downregulating TREM2 reversed the effect of circ-Epc1 on M2 microglial shifting during treatment with lipopolysaccharide (1 μg/mL). (A–D) Immunofluorescence detection of macrophage polarization using Iba-I+, CD11b+, and CD206+staining. Data are presented as the mean ± SEM. ***P < 0.001 vs. NC; ###P < 0.001 vs. circ-Epc1. (E–G) ELISA detection showing expression of the inflammatory factors, TNF-α, IL-6, and IL-1β. Data are presented the mean ± SD (n=10). ***P < 0.001 vs. NC; #P < 0.05 vs. circ-Epc1.