Research Paper Volume 14, Issue 7 pp 2989—3029

The tobacco phosphatidylethanolamine-binding protein NtFT4 increases the lifespan of Drosophila melanogaster by interacting with the proteostasis network

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Figure 2. Survival of Drosophila populations expressing NtFT4, NtFT2, CG7054 or CG7054dsRNA under the control of the daughterless (da) promoter. Survival curves of female (left) and male (right) flies in the filial generation after mating UAS-NtFT4, UAS-NtFT2, UAS-CG7054 or UASt-CG7054dsRNA with the da-Gal4 driver strain. (A, B) Effect on lifespan of flies constitutively expressing the floral inducer NtFT4 (A) or the floral repressor NtFT2 (B) compared with da-Gal4 x Oregon-R (n = 200). (C, D) Effect on lifespan of flies constitutively expressing the Drosophila PEBP CG7054 (C) or constitutively silencing CG7054 after mating UASt-CG7054dsRNA with the da-Gal4 driver strain (D) compared with da-Gal4 x Oregon-R (n = 200). Median and mean lifespans and statistical evaluation are summarized in Table 1 (female flies) and Supplementary Table 1 (male flies).