The tobacco phosphatidylethanolamine-binding protein NtFT4 increases the lifespan of <i>Drosophila melanogaster</i> by interacting with the proteostasis network

Philip K&#xE4;nel; Gundula A. Noll; Katrin Schroedter; Elke Naffin; Julia Kronenberg; Franziska Busswinkel; Richard M. Twyman; Christian Kl&#xE4;mbt; Dirk Pr&#xFC;fer

doi:doi:10.18632/aging.204005

← Back

Research Paper Volume 14, Issue 7 pp 2989—3029

The tobacco phosphatidylethanolamine-binding protein NtFT4 increases the lifespan of Drosophila melanogaster by interacting with the proteostasis network

Figure 3. Locomotor behavior of long-lived (da > NtFT4) or short-lived (da > CG7054^dsRNA) Drosophila populations at different ages. Rapid iterative negative geotaxis (RING) assay with virgin female (A) and male (B) flies at 10, 30 or 45 days old. The locomotor behavior was analyzed in the filial generation after mating Oregon-R (control, black), UAS-NtFT4 (green) or UASt-CG7054^dsRNA (blue) flies with the da-Gal4 driver strain. Negative geotaxis was plotted as average velocity (mm/10 s) and was traced for all tracks traveled in the population (numbers below plots). Significance was tested by one-way ANOVA and Tukey’s post hoc test between control, da > NtFT4 and da > CG7054^dsRNA (^****p < 0.001, Abbreviation: NS: not significant). All p-values are provided in Supplementary Table 9.