Research Paper Volume 14, Issue 7 pp 2989—3029

The tobacco phosphatidylethanolamine-binding protein NtFT4 increases the lifespan of Drosophila melanogaster by interacting with the proteostasis network


Figure 7. GeneChip 2.0 array and gene expression analysis of female flies expressing NtFT4. (A) Protein classes encoded by differentially expressed genes which were identified in the GeneChip Drosophila Genome 2.0 arrays (Affymetrix). We identified 149 genes that were significantly deregulated in female flies expressing NtFT4, 97 of which were mapped in the Panther database, and 63 genes were classified as representing 12 different protein classes. The largest protein classes were PC00262 (metabolite interconversion enzymes, 27 genes) and PC 00260 (protein modifying enzymes, 9 genes). Significance was determined using the paired t-test. Deregulated genes were included with a log2 fold change > 1.5 and a p-value < 0.05, n = 3. (BD) Gene expression analysis. Deregulated genes associated with proteolysis (CG1304, Ser6, CG31205, CG31681, CG32277, CG32523, Jon66Ci and Spn47C) (B), annotated as metabolic enzymes (C), or genes which cannot be classified into groups and genes of unknown function (non-classified/unknown function) (D) identified by transcriptome analysis were analyzed individually in 1d (left, blue), 5 d (middle, green) or 10 d (right, red) old female flies expressing NtFT4 (da > NtFT4) compared with control (da-Gal4) flies (black). Relative expression levels were calculated in relation to the reference genes Gapdh2, 14-3-3 ε and RpL32. Data are means ± SEM (n = 3), p-values are based on a t-test of pairwise comparisons between da > NtFT4 and da-Gal4 flies, ****p < 0.001, ***p < 0.01, **p < 0.05, *p < 0.1, Abbreviation: NS: not significant. The p-values of all comparisons are provided in Supplementary Table 9.