Research Paper Volume 14, Issue 7 pp 3313—3324
SNP rs322931 (C>T) in miR-181b and rs7158663 (G>A) in MEG3 aggravate the inflammatory response of anal abscess in patients with Crohn’s disease
Figure 6. The successful transfection of MEG3 siRNA, which was validated by the evidently reduced expression of MEG3 (A), promoted the expression of miR-181b (B) and repressed the expression of TNF-α mRNA (C) and protein (D). Moreover, the successful transfection of miR-181b precursors, which was validated by the significantly promoted miR-181b expression (B), suppressed the expression of MEG3 (A), TNF-α mRNA (C) and protein (D) (* P value < 0.05 vs. NC group; 3 biological repeats).