Research Paper Volume 14, Issue 10 pp 4486—4499

Long noncoding RNA KCNQ1OT1 inhibits osteoclast differentiation by regulating the miR-128-3p/NFAT5 axis

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Figure 1. KCNQ1OT1 suppresses the proliferation and migration of RAW 264.7 cells. (A) qRT-PCR for relative expression of KCNQ1OT1 in six bone tissues (three osteoporotic and three non-osteoporotic). (B) Relative expression of KCNQ1OT1 in RAW 264.7 cells transfected with pcDNA-KCNQ1OT1, si-KCNQ1OT1 or control vectors, as measured by qRT-PCR. (C) The proliferation curve and (D) transwell migration assays of RAW 264.7 cells transfected with pcDNA-KCNQ1OT1, si-KCNQ1OT1 or corresponding control vectors (Scale bar: 100 μm). *P < 0.05, ***P < 0.001, ns: not significant.