Research Paper Volume 14, Issue 12 pp 5086—5096

Galectin-3 induces vascular smooth muscle cells calcification via AMPK/TXNIP pathway


Figure 3. TXNIP mediated galectin-3-induced ROS and inflammation factors expression in VSMCs. VSMCs were treated with 10 μg/ml galectin-3 and (or) TXNIP siRNA for 24 h, The protein expression level of NLRP3, caspase-1, and IL-1β were measured by western blot, the results quantifications were shown in the right panel (A and B). Band density of native VSMCs was defined as a control and considered to 1. DHE staining was used to observe the ROS production (C). Data were obtained from three independent experiments. *P < 0.05, vs. the control; #P < 0.05 vs. galectin-3.