Research Paper Volume 14, Issue 12 pp 5177—5194

LncRNA PEG11as silencing sponges miR-874-3p to alleviate cerebral ischemia stroke via regulating autophagy in vivo and in vitro


Figure 5. PEG11as positively regulated ATG16L1expression by sponging miR-874-3p. (A) Possible binding sites of miR-874-3p in DDIT4 3’-UTR. (B) Bibiserv2 software was used to analyze 3’-UTR Hybrid energy analysis between miR-874-3p and ATG16L1. (C, D) The ATG16L1 3’-UTR-WT or ATG16L1 3’-UTR-MUT was determined after co-transfected with miR-765 mimic (C) or miR-874-3p inhibitor (D). (E) ATG16L1 protein level analyzed by western blot in the mouse brains treated by MCAO/R. (F) ATG16L1 protein level analyzed by western blot in N2a cells transfected with miR-874-3p mimic. One-way ANOVA followed by the Tukey’s post-hoc-test was used, data are shown as mean ± SD. Data are statistically different from each other with *P < 0.05, **P < 0.01, and ***P < 0.001.