Research Paper Volume 14, Issue 12 pp 4990—5012

Figure 4. Multiple copies of MEX67 or DBP5 rescue impaired RLS in sus1Δ cells. (A) Strategy used to identify specific genes that suppress sus1Δ defects. sus1Δ cells containing pRS316-SUS1 were transformed with the indicated pGP564 (LEU2)-based plasmids, including NPC-related genes. Cells were streaked on SC-Trp-His-Leu supplemented with 5-FOA twice to evict pRS316-SUS1. (B) Growth analysis of WT or sus1Δ strains transformed with the indicated plasmids, as described in Figure 1E. Each gene on the plasmids is listed in the right panel. (C) Schematic diagrams of the YGPM5d22 (top) and YGPM6e10 (bottom) plasmids. The ORF locations (arrows or boxes) and gene names are indicated. (D and E) RLS analysis of the indicated mutants (D) and sus1Δ cells transformed with the indicated plasmids (E). The RLS analysis in (E) was carried out on SC-leu plate. The mean lifespans are shown in parentheses. (F) Growth analysis of WT or sus1Δ strains transformed with the indicated plasmids, as described in Figure 1E. (G) RLS analysis of sus1Δ cells transformed with the indicated plasmids was carried out on SC plate. The mean lifespans are shown in parentheses.