Research Paper Volume 14, Issue 17 pp 6905—6916

NOX4 promotes Kupffer cell inflammatory response via ROS-NLRP3 to aggravate liver inflammatory injury in acute liver injury


Figure 5. Suppressing ROS inhibits inflammatory response and injury in KCs. (A, B) Results of FCM assay (n = 3). The cell apoptosis level in L/N + NAC group was significantly down-regulated, lower than that in L/N group. *P < 0.05, compared with Con group; #P < 0.05, compared with L/N group. (C) IF staining results (n = 3). NAC suppressed the activation of NLRP3 inflammasome, and the expression levels of NLRP3 and ASC markedly decreased, lower than those in L/N group. (D) ROS detection by DCFH-DA probe (n = 3). NAC suppressed ROS expression, and the number of positive cells evidently decreased, lower than that in L/N group.