**Figure 6.** **Cytotoxic agents and PAC-1 up-regulate apoptotic proteins BAD and BAX in MCDK cells.** (**A**–**D**) Arithmetic mean ± SEM of *BAD* transcript levels relative to *TBP* in MDCK cells incubated for 24 h without or with 10 μM cisplatin (**A**; *n* = 5; *paired t*-test), 120 nM paclitaxel (**B**; *n* = 5, *paired t*-test), 300 nM doxorubicin (**C**; *n* = 5, *paired t*-test), or 10 μM PAC-1 (**D**; *n* = 6, *paired t*-test). (**E**–**H**) Arithmetic mean ± SEM of BAX transcripts relative to TBP in MDCK cells treated without or with 10 μM cisplatin (**E**; *n* = 5, *Wilcoxon signed-rank* test), 120 nM paclitaxel (**F**; *n* = 5, *paired t*-test), 300 nM doxorubicin (**G**; *n* = 5, *Wilcoxon signed-rank* test), or 10 μM PAC-1 (**H**; *n* = 6, *paired t*-test). (**I**–**L**) Arithmetic mean ± SEM of BAX to BCL-2 mRNA ratio in MDCK cells incubated for 24 h without or with 10 μM cisplatin (**I**; *n* = 5, *paired t*-test), 120 nM paclitaxel (**J**; *n* = 5, *paired t*-test), 300 nM doxorubicin (**K**; *n* = 5, *paired* t-test), or 10 μM PAC-1 (**L**; *n* = 6, *paired t*-test). ^{*}*p* < 0.05, ^{**}*p* < 0.01, ^{***}*p* < 0.001 indicate significant difference from vehicle control; Abbreviations: a. u.: arbitrary units; ctr: control.