Impact of cytotoxic agents or apoptosis stimulants on αklotho in MDCK, NRK-52E and HK2 kidney cells

Figure 7.Cytotoxic agents and apoptosis inducers up-regulate PPARG in MDCK and NRK-52E cells. (A–E) Arithmetic mean ± SEM of PPARG transcript levels normalized to TBP in MDCK cells treated with or without 10 μM cisplatin (A; n = 5; paired t-test), 120 nM paclitaxel (B; n = 5; paired t-test), 300 nM doxorubicin (C; n = 5, paired t-test), 10 μM PAC-1 (D; n = 6, paired t-test), or with and without 5% FBS in the culture medium (E; n = 5, Wilcoxon signed-rank test) for 24 h. (F–J) Arithmetic mean ± SEM of PPARG mRNA levels relative to TBP in NRK-52E cells treated for 24 h with or without 10 μM cisplatin (F; n = 8; paired t-test), 120 nM paclitaxel (G; n = 5; paired t-test), 300 nM doxorubicin (H; n = 7, paired t-test), 10 μM PAC-1 (I; n = 6, paired t-test), or incubated with or without 5% NBCS in the culture medium (J; n = 6, Wilcoxon signed-rank test). ^{*}p < 0.05, ^{**}p < 0.01 indicate significant difference from vehicle control; Abbreviations: a. u.: arbitrary units; ctr: control.