Research Paper Volume 14, Issue 16 pp 6740—6755

Sulforaphane inhibits angiotensin II-induced cardiomyocyte apoptosis by acetylation modification of Nrf2


Figure 3. SFN up-regulates Nrf2 expression and function. Western blot (A), RT-qPCR (B) were used to detect Nrf2 protein and mRNA levels. Immunofluorescence staining was applied to detect p-Nrf2 expression and distribution (red) (C). Western blot was used to detect the expression of Nrf2 downstream antioxidant genes HO-1, NQO1, CAT (D). Data were presented as the mean SD (n = 3). *P < 0.05 vs control; # P < 0.05 vs Ang II.