Research Paper Volume 14, Issue 16 pp 6740—6755

Sulforaphane inhibits angiotensin II-induced cardiomyocyte apoptosis by acetylation modification of Nrf2


Figure 4. Nrf2 gene knockdown eliminated the preventive effect of SFN on Ang II-induced myocardial damage. Both Nrf2 knockdown cell lines and wild-type cell lines were given/not given Ang II, with/without SFN treatment. Western blot detected the expression of Nrf2 and downstream antioxidant genes NQO1 and HO-1 (A), TNF-α, 3-NT (B), cleaved caspase-3 (C). TUNEL staining detected apoptotic cells (D). Data were presented as the mean SD (n = 3). *P < 0.05 vs control; # P < 0.05 vs Ang II.