Research Paper Volume 14, Issue 16 pp 6740—6755
Sulforaphane inhibits angiotensin II-induced cardiomyocyte apoptosis by acetylation modification of Nrf2
Figure 5. SFN activates myocardial Nrf2 by enhancing the acetylation of histone H3 in Nrf2 promoter region. Immunofluorescent staining was used to detect Ace-H3 expression and distribution (red) (A). Western blot was used to detect Ace-H3 and Nrf2 expression (B). ChIP detected the enrichment of Ac- H3 in Nrf2 promoter region. (C) TSA, a deacetylase inhibitor, was used as a positive control. Data were presented as the mean SD (n = 3). *P < 0.05 vs control; # P < 0.05 vs Ang II.