Research Paper Volume 14, Issue 17 pp 6957—6974

Integrated PPI- and WGCNA-retrieval of hub gene signatures for soft substrates inhibition of human fibroblasts proliferation and differentiation


Figure 3. Soft substrates inhibited the proliferation of hFFs. (A) GSEA for cell cycle of mRNAs in soft/TCP. Enrichment score of cell cycle genes against their expression profile of soft/TCP. The x axis was the level, and the y axis was the enrichment score of these genes (left). Heat map showed the distribution of mRNAs expression of soft/TCP enriched under the cell cycle KEGG entry in all samples under the gene set, with each row representing one sample. Each line represented a gene, the color from blue to red represented mRNA expression from low to high (right). (B) Immunostaining against Ki67 of soft and TCP with or without TGF-β1 on day 1. Scale bars: 50 μm. (C) The positive rate of Ki67 in B. ***P < 0.001 (mean, n = 3 randomly selected fields from triplicate samples). (D) Detection of cell cycle by flow cytometry for soft and TCP with or without TGF-β1. (E) The percentage of PI. **P < 0.01; ***P < 0.001 (mean, n = 3). (F) The percentage of G1-, S-, and G2-phase in the cell cycle. *P < 0.05 vs. TCP; &&P < 0.01, &&&P < 0.001 vs. TCP+ TGF-β1 (mean, n = 3).