Research Paper Volume 14, Issue 17 pp 6957—6974

Integrated PPI- and WGCNA-retrieval of hub gene signatures for soft substrates inhibition of human fibroblasts proliferation and differentiation


Figure 4. Soft substrates inhibited contractility of hFFs. (A) GSEA for vascular smooth muscle contraction of mRNAs in soft/TCP. Enrichment score of vascular smooth muscle contraction genes against their expression profile of soft/TCP. The x axis was the level, and the y axis was the enrichment score of these genes (left). Heat map showed the distribution of mRNAs expression of soft/TCP enriched under the vascular smooth muscle contraction KEGG entry in all samples under the gene set, with each row representing one sample. Each line represented a gene, the color from blue to red represented mRNA expression from low to high (right). (B) Quantitative reverse transcription PCR (qRT-PCR) analysis comparing α-SMA expression levels in soft and TCP. ***P < 0.001 (mean, n = 3). (C) Immunostaining against PERIOSTIN and the positive rate on day 1. Scale bars: 50 μm. ***P < 0.001 (mean, n = 3 randomly selected fields from triplicate samples).