Research Paper Volume 14, Issue 17 pp 7156—7169

Combination of 7-O-geranylquercetin and microRNA-451 enhances antitumor effect of Adriamycin by reserving P-gp-mediated drug resistance in breast cancer


Figure 5. Combination of GQ and miR-451 inhibited the expression of MDR1 and P-gp. MCF-7/ADR cells and miR-451-transfected MCF-7/ADR cells were subjected to ADR (43 μM) or ADR (43 μM) combining GQ (10 μM) for 48 h. (A) Expression levels of MDR1 were detected by Real-time RT-PCR. The MCF-7/ADR cells only treated with ADR were regarded as control. (B) Expression levels of P-gp were detected by western blot. All data represent the means ± SD of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, comparing with ADR group; #P < 0.05, ###P < 0.001, comparing with miR-451, GQ and ADR combination group.