Research Paper Volume 14, Issue 20 pp 8179—8204

Crosstalk between age accumulated DNA-damage and the SIRT1-AKT-GSK3ß axis in urine derived renal progenitor cells


Figure 1. Comparative transcriptome and gene ontology analysis of urine-derived renal progenitors from young and aged donors. A hierarchical cluster dendrogram revealed distinct clusters of UdRPCs derived from young and aged donors (A). Expressed genes (det-p < 0.05) in UdRPCs and podocytes compared in the Venn diagram (B), shows distinct (750 in young and 155 in aged UdRPCs) and overlapping (11917) gene expression patterns. The most over- represented GO BP-terms exclusive in either young or old UdRPCs are shown in (C) and including DNA replication and renal development for the young UdRPCs and assembly of collagen fibrils and other multimeric structures and regulation of calcium ion transport for the old UdRPCs. The heatmap (D) reveals a distinct expression pattern between young and old UdRPCs for genes encoding for the following hallmarks of aging: genomic instability, epigenetic alterations, deregulation of nutrient sensing, cellular senescence, and stem cell exhaustion.