Research Paper Volume 14, Issue 18 pp 7568—7586

Novel TRKB agonists activate TRKB and downstream ERK and AKT signaling to protect Aβ-GFP SH-SY5Y cells against Aβ toxicity


Figure 4. Neuroprotective effects of LM-031 and analogs in Aβ-GFP SH-SY5Y cells. (A) Neurite outgrowth (length, process and branch) assay of Aβ-GFP cells uninduced, untreated, or treated with curcumin, LM-031 or analogs at 5 μM (n = 3). Shown below are images of TUBB3 (yellow)-stained cells, with nuclei counterstained with DAPI (blue), and segmented images with multi-colored mask to assign each outgrowth to a cell body for quantification. In uninduced cells, processes and branches are indicated with red and white arrows, respectively. (B) Caspase 1 and AChE activity assays with curcumin, LM-031 or analogs (5 μM) treatment (n = 3). The relative caspase 1 or AChE activity of uninduced cells (Dox-) was normalized (100%). P values: comparisons between induced (Dox+) vs. uninduced (Dox-) cells (##P < 0.01 and ###P < 0.001), or compound-treated vs. untreated (Dox+) cells (*P < 0.05, **P < 0.01, ***P < 0.001). (one-way ANOVA with post hoc Tukey test).