Research Paper Volume 14, Issue 20 pp 8346—8356

Figure 3. Silencing P50 inhibits the metastatic, invasive and glycolytic abilities of H22 cells. (A–C) In the DMSO-RNAi group, the invasive and metastatic abilities of cells weakened drastically, showing lower numbers of invasive and metastatic cells than in the DMSO group. ^*P < 0.05 vs. DMSO group. (D–F) Compared to the DMSO group, the DMSO-RNAi group exhibited significantly reduced glucose uptake, intracellular glucose level and lactate expression. ^*P < 0.05 vs. DMSO group. (G) Reduced number of clones formed was noted in the DMSO-RNAi group. (H, I) According to both HIF-1α IFA and Western-Blot results, the expression of HIF-1α could be suppressed after silencing P50. ^*P < 0.05 vs. DMSO group. (J, K) The number of Brdu-positive cells in the DMSO-RNAi group was less than that in the DMSO group, suggesting inhibition of the cell proliferation.