Research Paper Volume 15, Issue 1 pp 230—245
Increased expression of GPX4 promotes the tumorigenesis of thyroid cancer by inhibiting ferroptosis and predicts poor clinical outcomes
Figure 5. Knockdown of GPX4 inhibits proliferation in thyroid cancer cells. (A) GPX4 expression levels in FTC133, K1 and TPC-1 cells by western blot analysis. (B) GPX4 expression in FTC133 cells transfected with control, NC siRNA, or siGPX4 was confirmed by western blot analysis. (C) A CCK-8 assay was used to evaluate the viability of FTC133 cells transfected with NC siRNA/siGPX4. (D) Colony formation assay demonstrated the proliferation ability of FTC133 cells transfected with NC siRNA/siGPX4. (E) Ki-67 and PCNA protein expression in FTC133 cells transfected with NC siRNA/siGPX4 under erastin treatment.