Research Paper Volume 15, Issue 1 pp 246—260
Mitoquinone shifts energy metabolism to reduce ROS-induced oxeiptosis in female granulosa cells and mouse oocytes
Figure 2. Effects of MitoQ on imbalances in mitochondrial dynamics in ROS-exposed granulosa cells. (A) Mitochondrial network was stained with MitoTracker Green and analyzed using a fluorescence microscopy with image acquisition. (B) The images below were divided into six major categories based on the morphology of the mitochondria. (C) Three main mitochondrial types were quantified: fragmented, tubular and others. (D) The total length of each mitochondrion was assessed. (E) The levels of mitochondrial fission protein Drp1 were analyzed by immunoblotting. (F) Quantification of Drp1 phosphorylated protein levels. **p < 0.01 and ***p < 0.001.