Research Paper Volume 15, Issue 1 pp 21—36

The innate immune signaling component FBXC-58 mediates dietary restriction effects on healthy aging in Caenorhabditis elegans


Figure 1. Screen to uncover the innate immune signal mediating the dietary restriction (DR) effect. (A) Venn diagram depicting the genes overlapping between genes upregulated in DR-worms and upregulated in pathogen infected worms. (B) Relative expression level of each F-box gene between wild-type (N2) and eat-2(ad1116) mutant strains at day 2 of adulthood. (C) Relative expression levels of fbxc-58 mRNA in N2 on ad libitum (AL) and low density of food (LDF) condition at day 2 of adulthood. (D) Relative expression level of fbxc-58 in L4440 (L4440 RNAi N2) and fbxc-58 (fbxc-58 RNAi N2) at day 3 of adulthood. (E) (I) Schematic diagram for Pseudomonas aeruginosa (PA14) infection assay. (II) The survival rate of N2 L4440 (L4440 RNAi N2) and N2 fbxc-58 (fbxc-58 RNAi N2) in PA14 infection for 24 h at day 9 of adulthood. All of relative mRNA levels were determined by RT-PCR by three times independent experiments, normalized to act-3. Error bars represent SEM. Abbreviation: ns: not significant, *p < 0.05, **p < 0.01, ***p < 0.001; unpaired t test.