Research Paper Volume 15, Issue 1 pp 21—36

The innate immune signaling component FBXC-58 mediates dietary restriction effects on healthy aging in Caenorhabditis elegans


Figure 4. fbxc-58 is downstream target of ZIP-2 and PHA-4. (A) Relative expression levels of zip-2 mRNA in N2 L4440 RNAi (N2 L4440) and N2 zip-2 RNAi (N2 zip-2). (B) Relative expression levels of pha-4 mRNA in N2 L4440 RNAi (N2 L4440) and N2 pha-4 RNAi (N2 pha-4). (C) Relative expression level of fbxc-58 in N2 L4440 RNAi (N2 L4440), eat-2 L4440 RNAi (eat-2 L4440), eat-2 zip-2 RNAi (eat-2 zip-2), and eat-2 pha-4 RNAi (eat-2 pha-4) at day 3 of adulthood. (D) A schematic diagram of fbxc-58 induction mechanism in DR through ZIP-2 and PHA-4. All of relative mRNA levels were determined by RT-PCR by three times independent experiments, normalized to act-3. Error bars represent SEM. Abbreviation: ns: not significant, *p < 0.05, ***p < 0.001; unpaired t test.