Research Paper Volume 15, Issue 1 pp 21—36

The innate immune signaling component FBXC-58 mediates dietary restriction effects on healthy aging in Caenorhabditis elegans


Figure 5. fbxc-58 is downstream effector of S6Kinase signaling pathway. (A) Relative expression level of fbxc-58 in N2 and rsks-1(tm1714) mutant strains. (B) Relative expression level of pha-4 in N2 and rsks-1(tm1714) mutant strains. (C) Survival rate curves of N2 L4440 RNAi (n = 90), N2 fbxc-58 RNAi (n = 90), rsks-1 L4440 RNAi (n = 81), and rsks-1 fbxc-58 RNAi (n = 68). Survival data are summarized in Table 1. (D) A schematic diagram of fbxc-58 induction mechanism in DR through S6K, ZIP-2, and PHA-4. All of relative mRNA levels were determined by RT-PCR by three times independent experiments, normalized to act-3. Error bars mean SEM. *p < 0.05, **p < 0.01; unpaired t test.