Research Paper Volume 15, Issue 2 pp 492—512

Figure 5. M3541 treatment accelerates NK-cell dependent killing of irradiated cancer cells in vitro. (A) Growth/viability curves of A549 Nuclight Red cells exposed to DMSO, M3541, IR or IR+M3541 for 7 days, reseeded at equal number and co-cultured with NK cells from two healthy donors for 150 hours. Growth curves represent relative change in the number of viable cells (red-fluorescent nuclei) determined by continuous live imaging every 2h by IncuCyte. (B) Representative images of the above extracted from IncuCyte time lapse videos. (C) Relative expression of NK ligands, MICA, ULBP2, CD155 and ICAM1 in A549 exposed to DMSO, M3541, IR or IR+M3541 for 7 days, measured by quantitative PCR. Data represent mean ± SEM.