Research Paper Volume 15, Issue 4 pp 1025—1038

Circ_0001052 promotes cardiac hypertrophy via elevating Hipk3


Figure 3. Circ_0001052 competed with Hipk3 to interact with miR-148a-3p and miR-124-3p. (A) The subcellular location of circ_0001052. (B) RIP assay revealed the relative enrichment of circ_0001052 and Hipk3 in Ago2 or IgG group. (C) StarBase database predicted 15 miRNAs binding to both circ_0001052 and Hipk3. (D) RNA Pull down assay illustrated the relative enrichment of 15 candidate miRNAs pulled down by biotin labeled Hipk3. (E) The levels of miR-148a-3p/miR-124-3p in Ang II-treated CMs. (F) The overexpression efficiency of miR-148a-3p and miR-124-3p. (G) Influence of up-regulated miR-148a-3p or miR-124-3p on Hipk3 expression. (H) Binding sites between circ_0001052/Hipk3 and miR-148a-3p/miR-124-3p. (I) The luciferase activity of circ_0001052-WT/Mut or Hipk3-WT/Mut in response to the up-regulation of miR-148a-3p or miR-124-3p. (J) RIP assay tested the enrichment of circ_0001052, miR-148a-3p, miR-124-3p and Hipk3 in Ago2 or IgG group. **P < 0.01. Abbreviation: n.s: no significance.