Research Paper Volume 15, Issue 5 pp 1358—1370

Ginkgolide A improves the pleiotropic function and reinforces the neuroprotective effects by mesenchymal stem cell-derived exosomes in 6-OHDA-induced cell model of Parkinson’s disease


Figure 4. The therapeutic functions and characteristics of exosomes isolated from GA pre-treated WJMSCs. (A) Size distributions of exosomes as measured by NanoSight NS300 with mean values. (B) Exosome positive/negative markers TSG101, CD81, and Calnexin were measured by western blotting assay. (C) MTT assay was used to examine the SH-SY5Y cell viability. (D) Representative images of flow cytometry showed the cell apoptosis stained with Annexin V-FITC/PI apoptosis detection kit. (E) Statistical analysis of the apoptotic data from (D). (F) TUNEL assay was used to stain the DNA breaks to examine the therapeutic function of exosomes isolated from GA pre-treated WJMSCs. Data are presented as mean ± standard deviation. (G) TH staining after exosome treatment isolated from GA pre-treated WJMSCs under 6-OHDA damage. *P < 0.05 vs. control and #P < 0.05 vs. 6-OHDA groups.