Research Paper Volume 15, Issue 10 pp 4071—4083
lncRNA PITPNA-AS1 promotes cell proliferation and metastasis in hepatocellular carcinoma by upregulating PDGFD
Figure 3. PITPNA-AS1 sponges miR-363-3p in HCC cells. (A) A binding motif of miR-363-5p within PITPNA-AS1 predicted by StarBase3.0. (B) Relative mRNA level of miR-363-5p in four HCC cell lines (Huh7, BEL-7404, HCCLM3, and Hep3B) and the control cell line, THLE-3. (C) Luciferase activity in Huh7 and Hep3B cells transfected with miR-NC or miR-363-5p mimic. (D and E) Interaction between PITPNA-AS1 and miR-363-5p was determined by RIP and luciferase reporter assays. **P < 0.01.