Research Paper Volume 15, Issue 5 pp 1564—1590

Figure 4. miR-424 is associated with OS cell migration and invasion. (A) MiR-424 expression was lower in human OS than in chondroma tissue. Data represent the mean ± SD (n = 12). (B) MiR-424 expression was lower in human OS than in chondroma tissue. Representative images are shown. (C) MiR-424 expression in hFOB1.19 and OS cell lines (143B and HOS) was evaluated by RT-qPCR. Data represent the mean ± SD (n = 3). * P < 0.05. (D) MiR-424 overexpression did not affect circRBMS3 and RBMS3 expressions. (D) Pre-miR-424 or miR-424 sponge mediated miR-424 overexpression and inhibition of OS cell proliferation, as determined by the CCK-8 assay. Data are presented as the mean ± SD (n = 6). (E) MiR-424 overexpression suppressed cell growth, as determined by the colony formation assay (details are shown in the insets). Error bars represent the mean ± SD of 3 independent experiments. * P < 0.05. (F) HOS and 143B cells were transfected with miR-424 mimics, followed by Annexin V-FITC/PI staining. The percentage of apoptotic cells is shown as the mean ± SD from 3 independent experiments. * P < 0.05, significantly different compared with the vector group. (G) The effect of pre-miR-424 on cell migration capability was evaluated by a wound-healing assay in HOS and 143B cells. Data are the mean ± SD, n = 3. * P < 0.05. Scale bar, 200 μm. (H) Cell migration and invasion of HOS and 143B cells, transfected with pre-miR-424 or vector, were evaluated by transwell migration and Matrigel™ invasion assays. Data represent mean ± SD (n = 3). * P < 0.05. Scale bar, 200 μm.