Research Paper Volume 15, Issue 9 pp 3759—3770

LncRNA FALEC increases the proliferation, migration and drug resistance of cholangiocarcinoma through competitive regulation of miR-20a-5p/SHOC2 axis


Figure 1. The upregulated FALEC expression in cholangiocarcinoma (CCA) samples (n=20) and cell lines, the expression was analyzed by RT-PCR. (A, B) The mRNA levels of FALEC were up-regulated in CCA samples compared to adjacent normal tissues; (C) The mRNA levels of FALEC were not different in in CCA at different anatomical locations. (D) The mRNA levels of FALEC are significantly higher in patients who were resistant to 5-FU, compared to 5-FU sensitive patients; (E) The expressions of FALEC in 3 CCA cell lines were measured with and without 5-FU stimulation (100 μg/ml) compared with HIBEC cells. QBC939, and Huh-28 cell lines have higher FALEC expression and selected for further analysis; (F) IC50 of normally cultured CCA cells and their corresponding 5-FU resistant cells.