Research Paper Volume 15, Issue 9 pp 3759—3770

LncRNA FALEC increases the proliferation, migration and drug resistance of cholangiocarcinoma through competitive regulation of miR-20a-5p/SHOC2 axis


Figure 2. Knockdown FALEC increase the sensitivity of 5-FU resistant CCA cells. (A) FALEC level was significantly reduced in 5-FU resistant cell lines (QBC939-R and Huh-28-R) after transfected by si-FALEC. (B) Cell viability of QBC939-R and Huh-28-R decreased after transfected by si-FALEC; *, p<0.05, **, p<0.01, compared with negative control sequence (si-NC); (C) The proliferation ability of cells was measured by clone formation assay. Knocking down FALEC decreases the clone formation of QBC939-R and Huh-28-R significantly; (D) The migration ability of QBC939-R and Huh-28-R cells were measured by scratch method. Knocking down FALEC decreased the migration ability of both cell lines; (E) The invasion ability of QBC939-R and Huh-28-R cells decreased after knocking down FALEC.