Research Paper Volume 15, Issue 9 pp 3759—3770

LncRNA FALEC increases the proliferation, migration and drug resistance of cholangiocarcinoma through competitive regulation of miR-20a-5p/SHOC2 axis


Figure 3. FALEC suppresses miR-20a-5p expression by direct interaction. (A) Decreased miR-20a-5p expression in CCA tissue when compared to adjacent non-tumor tissues, and the expression was analyzed by RT-PCR; (B) The expression of miR-20a-5p increased after FALEC was blocked in QBC939-R and Huh-28-R cells; (C) The binding site between FALEC and miR-20a-5p was verified by dual luciferase reporter assay. The luciferase activity of the wild-type FALEC sequence (WT-FALEC) decreased significantly after the mimic sequence of miR-20a-5p was transfected into cells.