Research Paper Volume 15, Issue 9 pp 3759—3770

LncRNA FALEC increases the proliferation, migration and drug resistance of cholangiocarcinoma through competitive regulation of miR-20a-5p/SHOC2 axis


Figure 5. FALEC increased the 5-FU resistance of CCA cells by miR-20a-5p/SHOC2 axis. (A, B) Overexpression of FALEC (FALEC-OE) in normally cultured QBC939 and Huh-28 cells increased their resistance to 5-FU significantly in both cells; *, p<0.05, **, p<0.01, compared with blank control; #, p<0.05, ##, p<0.01, compared with FALEC-OE; (C, D) protein expression of SHOC2 and its downstream activator protein pERK1/2 increased after enhancing FALEC expression (FALEC-OE) in both cell lines. Upregulation of miR-20a-5p or knockdown SHOC2 (si-SHOC2) abolished the enhancing effect to SHOC2, and pERK1/2 caused by FALEC.